The player unlocks Mastery mode for Beginner tracks by earning at least one medal on 39 tracks and all medals (except the Ten medal) on 17 Beginner tracks. Other Track Types require unlocking the previous Mastery level and having all medals on more tracks. Each Mastery level unlocked also unlocks an extra tier of upgrades in the Monkey Lab.
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Notable rounds
Leads first appear on round 22.
Ceramics first appear on round 35.
Seven Camo Leads appear on round 37, and nowhere else.
Four MOABs appear on round 40.
A BFB appears on round 46.
A ZOMG appears on round 60, and two appear on round 65.
Rounds 63, 76, and 78 have tightly packed MOABs instead of Ceramics.
Gallery
Screenshots
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Medals
Easy
Medium
Hard
Impoppable
Reverse
Ten
Trivia
Internally, Mastery mode simply adds 1 to each Bloon's rank. As a result, round 85 is completely unchanged.
Additionally, when the Golden Bloon was released, it was considered 1 rank above the Ceramic Bloon, causing a bug where Ceramics were upgraded to Golden Bloons instead of MOABs.
References
↑"Do you have what it takes to become the ultimate Bloons master? Prove it with the brand new Masteries mode!" — In-game description of the 3.0 update.