One of the Green or Yellow Bloons in the double helix or one of the Red Bloons on the sides.
Golden Bloon
After the spinning Red Bloons to the left and right of the double helix appear, it enters from the top-right and moves down in a sine wave.
Mystery Bloon 3
One of the Black Bloons, Yellow Bloons, or Blue Bloons in the windmill.
Strategy
First time
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Diamond medal
The Diamond medal can easily be earned by equipping Radar Glaives without any powerups, and sitting below the screen. Other weapons can be used if the player wants the diamond medal earlier in progression, as there are very few bloons.